Genome editing of LKB1 (STK11) mutation in A549 lung cancer cells using CRISPR/Cas9 system
نویسندگان
چکیده
منابع مشابه
Dihydroartemisinin increases radiosensitivity of A549 lung cancer cells
Background: Radiotherapy is the gold standard in the treatment of lung cancer. However, the radiosensitization of cancerous cells requires further improvement. Here, we investigated the effect of dihydroartemisinin (DHA) on the radiosensitization of non-small cell lung cancer (NSCLC) cells. Methods: Cell proliferation and cell cycle assays were carried out using A549 cells exposed to DHA. The e...
متن کاملLKB1/STK11 suppresses cyclooxygenase-2 induction and cellular invasion through PEA3 in lung cancer.
We showed that the PEA3 transcriptional factor interacted with LKB1, a serine/threonine kinase, which is somatically mutated in lung cancer. This interaction occurred through the ETS domain of PEA3 and the kinase domain of LKB1. Mutation of LKB1 in lung cancer cells stabilized PEA3. Reintroduction of wild-type (WT) LKB1 into cells induced down-regulation of PEA3 and subsequently resulted in red...
متن کاملATM induces radioresistance of non-small cell lung cancer A549 cells by downregulation of MDMX
Background: Tumor radioresistance leads to a reduction in the efficiency of radiation therapy. It is very important to explore the cellular mechanisms leading to radioresistance and to find potential therapeutic targets, which might improve the efficacy of radiation therapy. This study was to investigate the role of ataxia-telangiectasia mutated (ATM) and murine double minute X (MDMX) in radior...
متن کاملAnalysis of Stk11/lkb1 Gene Using Bioinformatics Tools
Aim: The STK11 gene, also known as LKB1, encodes for a serine/threonine kinase with growth-suppressing activity, such as inhibition of cell cycle progression, cell growth retardation, apoptotic cell death, and cell polarity control. This study aimed to investigate some properties of the STK11 gene and its product, such as the homologous protein sequences in different species, the common transcr...
متن کاملInactivation of LKB1/STK11 is a common event in adenocarcinomas of the lung.
Frequent losses of chromosome 19p have recently been observed in sporadic lung adenocarcinomas, targeting the location of a critical tumor suppressor gene. Here we performed fine mapping of the short arm of chromosome 19 and found that the LKB1/STK11 gene mapped in the minimal-deleted region. Because germ-line mutations at LKB1/STK11 result in the Peutz-Jeghers syndrome and an increased risk of...
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ژورنال
عنوان ژورنال: Revista dos Trabalhos de Iniciação Científica da UNICAMP
سال: 2018
ISSN: 2596-1969
DOI: 10.20396/revpibic262018138